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Mouse Anti-CRISPR Cas9 Recombinant Antibody (6H4) (CBMAB-BR061LY)


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Summary

Host Animal
Mouse
Specificity
Streptococcus pyogenes
Clone
6H4
Antibody Isotype
IgG2b
Application
IP, IF, WB

Basic Information

Immunogen
N-terminal region, amino acids 1-608 of Cas9 sequence CDJ55032.1 from Streptococcus pyogenes, expressed in and purified from E. coli.
Host Species
Mouse
Specificity
Streptococcus pyogenes
Antibody Isotype
IgG2b
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:200
IF(ICC)1:400-1:1,600

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CRISPR-associated endonuclease Cas9
UniProt ID
G3ECR1
Alternative Names
CRISPR-associated endonuclease Cas9 ;3.1.-.- ;St-Cas9;cas9 ;csn1;
Function
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids) (PubMed:21455174).
CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA:tracrRNA interaction and has no catalytic function in RNA processing (PubMed:24270795).
Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed 3'-5' exonucleolytically. DNA-binding requires protein and both gRNAs, as does nuclease activity. Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. DNA strand separation and heteroduplex formation starts at PAM sites; PAM recognition is required for catalytic activity (PubMed:24476820).
Confers immunity against a plasmid with homology to the appropriate CRISPR spacer sequences (CRISPR interference) (PubMed:21455174).
Biological Process
Defense response to virus Source: UniProtKB-UniRule
Maintenance of CRISPR repeat elements Source: UniProtKB
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For research use only. Not intended for any clinical use.

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