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Mouse Anti-CARD10 Recombinant Antibody (EG478) (CBMAB-EN542-LY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Pig
Clone
EG478
Antibody Isotype
IgG3, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 857-877 near the C-terminus of CARD 10 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Pig
Antibody Isotype
IgG3, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Caspase Recruitment Domain Family Member 10
Introduction
The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008]
Entrez Gene ID
Human29775
Mouse105844
UniProt ID
HumanQ9BWT7
MouseP58660
Alternative Names
Caspase Recruitment Domain Family Member 10; CARD-Containing MAGUK Protein 3; Carma 3; CARMA3; Caspase Recruitment Domain-Containing Protein 10; Caspase Recruitment Domain Family, Member 10; Bcl10 Binding Protein And Activator Of NFKB; CARD-Containing MAGUK 3 Protein; BIMP1;
Function
Activates NF-kappa-B via BCL10 and IKK.
Biological Process
Activation of NF-kappaB-inducing kinase activity Source: UniProtKB
Negative regulation of cell migration involved in sprouting angiogenesis Source: BHF-UCL
Positive regulation of protein localization to nucleus Source: BHF-UCL
Protein-containing complex assembly Source: UniProtKB
Regulation of apoptotic process Source: InterPro
Cellular Location
Cytoplasm
More Infomation

Israël, L., Glück, A., Berger, M., Coral, M., Ceci, M., Unterreiner, A., ... & Bornancin, F. (2021). CARD10 cleavage by MALT1 restricts lung carcinoma growth in vivo. Oncogenesis, 10(4), 1-11.

Yang, D. H., Guo, T., Yuan, Z. Z., Lei, C., Ding, S. Z., Yang, Y. F., ... & Luo, H. (2020). Mutant CARD10 in a family with progressive immunodeficiency and autoimmunity. Cellular & molecular immunology, 17(7), 782-784.

Zhang, C., Wang, H., & Yang, B. (2020). miR-146a regulates inflammation and development in patients with abdominal aortic aneurysms by targeting CARD10. International angiology: a journal of the International Union of Angiology.

Peng, L., He, K., Cao, Z., Bi, L., Yu, D., Wang, Q., & Wang, J. (2020). CARD10 promotes the progression of renal cell carcinoma by regulating the NF‑κB signaling pathway. Molecular medicine reports, 21(1), 329-337.

Motawi, T. K., El-Maraghy, S. A., Sharaf, S. A., & Said, S. E. (2019). Association of CARD10 rs6000782 and TNF rs1799724 variants with paediatric-onset autoimmune hepatitis. Journal of advanced research, 15, 103-110.

Liu, X., Zhang, X., Bi, J., Li, Z., Zhang, Z., & Kong, C. (2019). Caspase recruitment domain family member 10 regulates carbamoyl phosphate synthase 1 and promotes cancer growth in bladder cancer cells. Journal of cellular and molecular medicine, 23(12), 8128-8138.

Israël, L., Bardet, M., Huppertz, A., Mercado, N., Ginster, S., Unterreiner, A., ... & Bornancin, F. (2018). A CARD10-dependent tonic signalosome activates MALT1 paracaspase and regulates IL-17/TNF-α-driven keratinocyte inflammation. The Journal of investigative dermatology, 138(9), 2075-2079.

Yamamoto-Furusho, J. K., Fonseca-Camarillo, G., Furuzawa-Carballeda, J., Sarmiento-Aguilar, A., Barreto-Zuñiga, R., Martínez-Benitez, B., & Lara-Velazquez, M. A. (2018). Caspase recruitment domain (CARD) family (CARD9, CARD10, CARD11, CARD14 and CARD15) are increased during active inflammation in patients with inflammatory bowel disease. Journal of Inflammation, 15(1), 1-12.

Zou, Y., Zhou, J. Y., Wang, F., Zhang, Z. Y., Liu, F. Y., Luo, Y., ... & Huang, O. P. (2018). Analysis of CARD10 and CARD11 somatic mutations in patients with ovarian endometriosis. Oncology letters, 16(1), 491-496.

Shyamsunder, P., Sankar, H., Mayakonda, A., Han, L., Nordin, H. B. M., Woon, T. W., ... & Koeffler, H. P. (2018). CARD10, a CEBPE target involved in granulocytic differentiation. haematologica, 103(8), 1269.

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For research use only. Not intended for any clinical use.

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