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Mouse Anti-CAPN9 Recombinant Antibody (3A6) (CBMAB-C5658-LY)


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Published Data
CBMAB-C5658-LY-1
Mouse Anti-CAPN9 Recombinant Antibody (3A6) (CBMAB-C5658-LY) in IHC
Representative photomicrographs following immunohistochemical staining of (A) high calpain-9 and (B) low calpain-9 staining. Photomicrographs are shown at 100x magnification with 200x magnification inset box where scale shows 100 μm. ...View More
Davis, J., Martin, S. G., Patel, P. M., Green, A. R., Rakha, E. A., Ellis, I. O., & Storr, S. J. (2014). Low calpain-9 is associated with adverse disease-specific survival following endocrine therapy in breast cancer. BMC cancer, 14, 1-8.
CBMAB-C5658-LY-2
Mouse Anti-CAPN9 Recombinant Antibody (3A6) (CBMAB-C5658-LY) in WB
Western blot analysis of mouse tissues using antibodies against calpain 8, calpain 9, calpain 1, calpain 2, and β-actin. Twenty micrograms of tissue homogenate was used for each lane except for β-actin, for which 2 µg of homogenate was used. Lanes: 1, gastric mucosa; 2, jejunum; 3, colon; 4, spleen; 5, liver; 6, kidney; 7, lung; 8, uterus; 9, heart; 10, brain. Asterisks indicate non-specific signals originating from the secondary antibody. ...View More
Hata, S., Abe, M., Suzuki, H., Kitamura, F., Toyama-Sorimachi, N., Abe, K., ... & Sorimachi, H. (2010). Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense. PLoS genetics, 6(7), e1001040.
CBMAB-C5658-LY-3
Mouse Anti-CAPN9 Recombinant Antibody (3A6) (CBMAB-C5658-LY) in IF
Double-immunostaining of the mouse stomach was performed using antibodies against calpain 8 (red) and calpain 9 (green). The right panel is a magnified view of the boxed area. Blue signals represent DAPI-stained nuclei. Bars, 50 µm. ...View More
Hata, S., Abe, M., Suzuki, H., Kitamura, F., Toyama-Sorimachi, N., Abe, K., ... & Sorimachi, H. (2010). Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense. PLoS genetics, 6(7), e1001040.
CBMAB-C5658-LY-4
Mouse Anti-CAPN9 Recombinant Antibody (3A6) (CBMAB-C5658-LY) in IHC
The gastric mucosa of Capn8−/−, Capn9−/−, and WT mice was sectioned and analyzed by hematoxylin-eosin (H-E) staining (A), and periodic acid-Schiff (PAS) staining (B). Bars, 100 µm. ...View More
Hata, S., Abe, M., Suzuki, H., Kitamura, F., Toyama-Sorimachi, N., Abe, K., ... & Sorimachi, H. (2010). Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense. PLoS genetics, 6(7), e1001040.
CBMAB-C5658-LY-5
Mouse Anti-CAPN9 Recombinant Antibody (3A6) (CBMAB-C5658-LY) in IF
Immunostaining of WT, Capn8−/−, and Capn9−/− mouse stomachs before (a-c) and after (d-f) ethanol administration using an antibody for Muc5AC (green). Bars, 50 µm. ...View More
Hata, S., Abe, M., Suzuki, H., Kitamura, F., Toyama-Sorimachi, N., Abe, K., ... & Sorimachi, H. (2010). Calpain 8/nCL-2 and calpain 9/nCL-4 constitute an active protease complex, G-calpain, involved in gastric mucosal defense. PLoS genetics, 6(7), e1001040.

Summary

Host Animal
Mouse
Specificity
Human
Clone
3A6
Antibody Isotype
IgG2a, κ
Application
ELISA, IHC, WB

Basic Information

Immunogen
Partial recombinant corresponding to aa591-690 from human CAPN9 with GST tag.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
calpain 9
Introduction
Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Entrez Gene ID
10753
UniProt ID
O14815
Alternative Names
Calpain 9; Digestive Tract-Specific Calpain; Novel Calpain Large Subunit-4; New Calpain 4; NCL-4; Calpain 9 (NCL-4); Protein CG36;
Function
Calcium-regulated non-lysosomal thiol-protease.
Biological Process
Digestion Source: ProtInc
Proteolysis Source: GO_Central
Cellular Location
Cytoplasm
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For research use only. Not intended for any clinical use.

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