Mouse Anti-CAPN6 Recombinant Antibody (CBFYC-0799) (CBMAB-C0854-FY)

Name: Mouse Anti-CAPN6 Recombinant Antibody (CBFYC-0799)
SKU: CBMAB-C0854-FY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYC-0799

Antibody Isotype

IgG1

Application

WB, IP, FC

Basic Information

Immunogen

Recombinant protein corresponding to Calpain 6 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Calpain 6

Introduction

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is highly expressed in the placenta. Its C-terminal region lacks any homology to the calmodulin-like domain of other calpains. The protein lacks critical active site residues and thus is suggested to be proteolytically inactive. The protein may play a role in tumor formation by inhibiting apoptosis and promoting angiogenesis.

Entrez Gene ID

12338

UniProt ID

O35646

Alternative Names

calpain-6

Function

Microtubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues (By similarity).

Biological Process

Microtubule bundle formation Source: UniProtKB
Regulation of cytoskeleton organization Source: UniProtKB

Cellular Location

Spindle; Perinuclear region. During mitose associated with the mitotic spindle. At telophase colocalized to the midbody spindle.

More Infomation

References

Zhang, Y. Y., Gu, L. J., Zhu, N., Wang, L., Cai, M. C., Jia, J. S., ... & Yuan, W. J. (2021). Calpain 6 inhibits autophagy in inflammatory environments: A preliminary study on myoblasts and a chronic kidney disease rat model. International journal of molecular medicine, 48(4), 1-10.

Mamoor, S. (2020). Calpain 6, encoded by CAPN6 is differentially expressed in metastatic breast cancer, both in metastases to the brain and to the lymph nodes.

Zhu, L., Sun, Y., Wu, Q., Zhang, C., & Ling, J. (2020). CAPN6 regulates uterine leiomyoma cell proliferation and apoptosis through the Rac1-dependent signaling pathway. Annals of Clinical & Laboratory Science, 50(1), 24-30.

Chen, L., Xiao, D., Tang, F., Gao, H., & Li, X. (2020). CAPN6 in disease: An emerging therapeutic target. International Journal of Molecular Medicine.

Kim, B. H., Do Yeon Kim, S. O., Ko, J. Y., Rah, G., Yoo, K. H., & Park, J. H. (2019). Deficiency of calpain-6 inhibits primary ciliogenesis. BMB reports, 52(10), 619.

Oh, M., Rho, S. B., Son, C., Park, K., & Song, S. Y. (2019). Non-proteolytic calpain-6 interacts with VEGFA and promotes angiogenesis by increasing VEGF secretion. Scientific reports, 9(1), 1-11.

Xiang, Y., Li, F., Wang, L., Zheng, A., Zuo, J., Li, M., ... & Tao, Z. (2017). Decreased calpain 6 expression is associated with tumorigenesis and poor prognosis in HNSCC. Oncology letters, 13(4), 2237-2243.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

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SDS
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