Mouse Anti-CACNB4 Recombinant Antibody (CBXC-3048) (CBMAB-C3629-CQ)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

CBXC-3048

Antibody Isotype

IgG2a, κ

Application

WB, IP, IF, E

Basic Information

Immunogen

Amino acids 425-463 near the C-terminus of L-type Ca++ CP β4 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Calcium Voltage-Gated Channel Auxiliary Subunit Beta 4

Introduction

This gene encodes a member of the beta subunit family of voltage-dependent calcium channel complex proteins. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein encoded by this locus plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE), juvenile myoclonic epilepsy (JME), and episodic ataxia, type 5.

Entrez Gene ID

785

UniProt ID

O00305

Alternative Names

Calcium Voltage-Gated Channel Auxiliary Subunit Beta 4; Calcium Channel Voltage-Dependent Subunit Beta 4; CACNLB4; CAB4; Dihydropyridine-Sensitive L-Type, Calcium Channel Beta-4 Subunit; Voltage-Dependent L-Type Calcium Channel Subunit Beta-4; Calcium Channel, Voltage-Dependent, Beta 4 Subunit;

Function

The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.

Biological Process

Chemical synaptic transmission Source: GO_Central
Membrane depolarization Source: Reactome
Neuromuscular junction development Source: GO_Central
Regulation of voltage-gated calcium channel activity Source: UniProtKB

Cellular Location

Cytosol; Cytoplasmic side of plasma membrane; Plasma membrane; Voltage-gated calcium channel complex; Synapse

Involvement in disease

Epilepsy, idiopathic generalized 9 (EIG9): A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain.
Juvenile myoclonic epilepsy 6 (EJM6): A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.
Episodic ataxia 5 (EA5): A disorder characterized by episodes of vertigo and ataxia that last for several hours. Interictal examination show spontaneous downbeat and gaze-evoked nystagmus, mild dysarthria and truncal ataxia.

More Infomation

References

Coste de Bagneaux, P., von Elsner, L., Bierhals, T., Campiglio, M., Johannsen, J., Obermair, G. J., ... & Kutsche, K. (2020). A homozygous missense variant in CACNB4 encoding the auxiliary calcium channel beta4 subunit causes a severe neurodevelopmental disorder and impairs channel and non-channel functions. PLoS genetics, 16(3), e1008625.

Rima, M., Daghsni, M., Lopez, A., Fajloun, Z., Lefrancois, L., Dunach, M., ... & Ronjat, M. (2017). Down-regulation of the Wnt/β-catenin signaling pathway by Cacnb4. Molecular biology of the cell, 28(25), 3699-3708.

Rima, M., Daghsni, M., De Waard, S., Gaborit, N., Fajloun, Z., Ronjat, M., ... & De Waard, M. (2017). The β4 subunit of the voltage-gated calcium channel (Cacnb4) regulates the rate of cell proliferation in Chinese Hamster Ovary cells. The international journal of biochemistry & cell biology, 89, 57-70.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-CACNB4 Recombinant Antibody (7E1)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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