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Mouse Anti-BTRC Recombinant Antibody (CBYY-0931) (CBMAB-0934-YY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-0931
Antibody Isotype
IgG1
Application
WB, IF, E

Basic Information

Immunogen
Purified recombinant fragment of human beta-TrCP1/BTRC (AA: 24-151) expressed in E. coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:2,000
ELISA1:100-1:2,000
IHC1:10-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Beta-Transducin Repeat Containing E3 Ubiquitin Protein Ligase
Introduction
This gene encodes a member of the F-Bovinex protein family which is characterized by an approximately 40 amino acid motif, the F-Bovinex. The F-Bovinex proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-Bovinex), which function in phosphorylation-dependent ubiquitination. The F-Bovinex proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-Bovinex, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar 2012]
Entrez Gene ID
8945
UniProt ID
Q9Y297
Alternative Names
Beta-Transducin Repeat Containing E3 Ubiquitin Protein Ligase; F-Bovinex And WD Repeats Protein Beta-TrCP; PIkappaBalpha-E3 Receptor Subunit; Epididymis Tissue Protein Li 2a; E3RSIkappaB; FBXW1A; BTrCP; FBW1A; F-Bovinex/WD Repeat-Containing Protein 1A;
Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins (PubMed:10066435, PubMed:10497169, PubMed:10644755, PubMed:10835356, PubMed:11238952, PubMed:11359933, PubMed:11994270, PubMed:12791267, PubMed:12902344, PubMed:14603323, PubMed:14681206, PubMed:14988407, PubMed:15448698, PubMed:15917222, PubMed:16371461, PubMed:25503564, PubMed:25704143, PubMed:9859996, PubMed:22087322).
SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling (PubMed:12077367, PubMed:12820959).
SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1, ATF4, CDC25A, DLG1, FBXO5, PER1, SMAD3, SMAD4, SNAI1 and probably NFKB2 (PubMed:10835356, PubMed:11238952, PubMed:14681206, PubMed:14603323).
SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription (PubMed:10066435, PubMed:10497169, PubMed:10644755).
Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22' (PubMed:10066435).
SCF(BTRC) mediates the ubiquitination of CEP68; this is required for centriole separation during mitosis (PubMed:25704143, PubMed:25503564).
SCF(BTRC) mediates the ubiquitination and subsequent degradation of nuclear NFE2L1 (By similarity).
Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and PER2 (PubMed:15917222).
May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3 (PubMed:16371461).
Mediates ubiquitination of REST, thereby leading to its proteasomal degradation (PubMed:21258371, PubMed:18354482).
Biological Process
Branching involved in mammary gland duct morphogenesis Source: Ensembl
Cellular response to organic cyclic compound Source: Ensembl
Fc-epsilon receptor signaling pathway Source: Reactome
G2/M transition of mitotic cell cycle Source: Reactome
Interleukin-1-mediated signaling pathway Source: Reactome
Mammary gland epithelial cell proliferation Source: Ensembl
Negative regulation of DNA-binding transcription factor activity Source: BHF-UCL
Negative regulation of smoothened signaling pathway Source: BHF-UCL
Negative regulation of transcription, DNA-templated Source: UniProtKB
NIK/NF-kappaB signaling Source: Reactome
Positive regulation of circadian rhythm Source: UniProtKB
Positive regulation of proteolysis Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of ubiquitin protein ligase activity Source: ParkinsonsUK-UCL
Post-translational protein modification Source: Reactome
Proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
Protein dephosphorylation Source: UniProtKB
Protein destabilization Source: UniProtKB
Protein K48-linked ubiquitination Source: Ensembl
Protein polyubiquitination Source: ParkinsonsUK-UCL
Protein ubiquitination Source: UniProtKB
Regulation of canonical Wnt signaling pathway Source: ParkinsonsUK-UCL
Regulation of circadian rhythm Source: UniProtKB
Regulation of I-kappaB kinase/NF-kappaB signaling Source: Ensembl
Regulation of mitotic cell cycle phase transition Source: Reactome
Regulation of proteasomal protein catabolic process Source: Ensembl
Rhythmic process Source: UniProtKB-KW
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Signal transduction Source: ProtInc
Stimulatory C-type lectin receptor signaling pathway Source: Reactome
Stress-activated MAPK cascade Source: Reactome
T cell receptor signaling pathway Source: Reactome
Ubiquitin-dependent protein catabolic process Source: UniProtKB
Viral process Source: UniProtKB-KW
Wnt signaling pathway Source: Reactome
Cellular Location
Cytoplasm; Nucleus
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For research use only. Not intended for any clinical use.

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