Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-BPI Recombinant Antibody (CBYY-0736) (CBMAB-0739-YY)


See all BPI antibodies
Online Inquiry
Published Data
CBMAB-0739-YY-1
CBMAB-0739-YY in Immunoblot
Cell-free supernatants were collected at 10 min and immunoblotted for markers of tertiary granule (MMP-9), secondary granule (hCAP-18), or primary granule (BPI) release. ...View More
Fee, L. T., Gogoi, D., O'Brien, M. E., McHugh, E., Casey, M., Gough, C., ... & Reeves, E. P. (2021). C3d elicits neutrophil degranulation and decreases endothelial cell migration, with implications for patients with alpha-1 antitrypsin deficiency. Biomedicines, 9(12), 1925.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-0736
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, E

Basic Information

Immunogen
Amino acids 229-253 to the elastase cleavage site of BPI of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Bactericidal/Permeability-Increasing Protein
Introduction
This gene encodes a lipopolysaccharide binding protein. It is associated with human neutrophil granules and has antimicrobial activity against gram-negative organisMouse.
Entrez Gene ID
671
UniProt ID
P17213
Alternative Names
Bactericidal/Permeability-Increasing Protein; BPI Fold Containing Family D, Member 1; CAP 57; Bactericidal Permeability-Increasing Protein; Recombinant BPI Holoprotein, RBPI; BPIFD1; RBPI;
Function
The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.
Biological Process
Antibacterial humoral response Source: UniProtKB
Antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
Antimicrobial humoral response Source: Reactome
Cellular response to lipopolysaccharide Source: UniProtKB
Defense response to Gram-negative bacterium Source: UniProtKB
Innate immune response Source: GO_Central
Lipopolysaccharide-mediated signaling pathway Source: GO_Central
Negative regulation of interleukin-6 production Source: BHF-UCL
Negative regulation of interleukin-8 production Source: BHF-UCL
Negative regulation of macrophage activation Source: BHF-UCL
Negative regulation of tumor necrosis factor production Source: BHF-UCL
Neutrophil degranulation Source: Reactome
Cellular Location
Secreted; Cytoplasmic granule membrane. Membrane-associated in polymorphonuclear Leukocytes (PMN) granules.
More Infomation

Theprungsirikul, J., Skopelja-Gardner, S., Burns, A. S., Wierzbicki, R. M., & Rigby, W. F. (2021). Bactericidal/Permeability-Increasing Protein Preeminently Mediates Clearance of Pseudomonas aeruginosa In Vivo via CD18-Dependent Phagocytosis. Frontiers in immunology, 12, 1407.

Theprungsirikul, J., Skopelja-Gardner, S., Wierzbicki, R. M., Sessions, K. J., & Rigby, W. F. (2021). Differential Enhancement of Neutrophil Phagocytosis by Anti–Bactericidal/Permeability-Increasing Protein Antibodies. The Journal of Immunology, 207(3), 777-783.

Liu, Y., Zha, H., Han, X., Yu, S., Chai, Y., Zhong, J., & Zhu, Q. (2021). Molecular characterization and functional analysis of the bactericidal permeability-increasing protein/LPS-binding protein (BPI/LBP) from roughskin sculpin (Trachidermus fasciatus). Developmental & Comparative Immunology, 123, 104133.

Kong, Q., Lv, Z., Kang, Y., An, Y., Liu, Z., & Zhang, J. (2021). Bactericidal Permeability Increasing Protein Deficiency Aggravates Acute Colitis in Mice by Increasing the Serum Levels of Lipopolysaccharide. Frontiers in Immunology, 11, 3543.

Theprungsirikul, J., Thaden, J. T., Wierzbicki, R. M., Burns, A. S., Skopelja-Gardner, S., Fowler Jr, V. G., ... & Rigby, W. F. C. (2020). Low-avidity autoantibodies against bactericidal/permeability-increasing protein occur in gram-negative and gram-positive bacteremia. Infection and immunity, 88(10), e00444-20.

McQuillan, K., Gargoum, F., Murphy, M. P., McElvaney, O. J., McElvaney, N. G., & Reeves, E. P. (2020). Targeting IgG Autoantibodies for Improved Cytotoxicity of Bactericidal Permeability Increasing Protein in Cystic Fibrosis. Frontiers in Pharmacology, 11, 1098.

Yang, D., Han, Y., Chen, L., Cao, R., Wang, Q., Dong, Z., ... & Zhao, J. (2019). A bactericidal permeability-increasing protein (BPI) from manila clam Ruditapes philippinarum: Investigation on the antibacterial activities and antibacterial action mode. Fish & shellfish immunology, 93, 841-850.

Skopelja-Gardner, S., Theprungsirikul, J., Meagher, R. E., Beliveau, C. M., Bradley, K. E., Avery, M., ... & Rigby, W. F. (2019). Autoimmunity to bactericidal/permeability-increasing protein in bronchiectasis exhibits a requirement for Pseudomonas aeruginosa IgG response. European Respiratory Journal, 53(2).

Bülow, S., Zeller, L., Werner, M., Toelge, M., Holzinger, J., Entzian, C., ... & Gessner, A. (2018). Bactericidal/permeability-increasing protein is an enhancer of bacterial lipoprotein recognition. Frontiers in immunology, 9, 2768.

Balakrishnan, A., & Chakravortty, D. (2017). Epithelial cell damage activates bactericidal/permeability increasing-protein (BPI) expression in intestinal epithelium. Frontiers in microbiology, 8, 1567.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-BPI Recombinant Antibody (CBYY-0736)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry