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Mouse Anti-Bora Recombinant Antibody (CBYY-0726) (CBMAB-0729-YY)


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Published Data
CBMAB-0729-YY-1
CBMAB-0729-YY in WB
Interphase extracts were immunodepleted using anti-Bora antibodies and 30 min later supplemented with a recombinant hyperactive GwlK72M and Bora1-224 (lanes 2-5), Bora35-157 (lanes 6-9), Bora1-224 (lanes 11-14), Bora18-157 [S27A T52A] (lanes 15-18), Bora18-120 [S27A T52A] (lanes 19-22). A fraction of the extracts was collected at the indicated time-points (0, 20, 40, 60 min) and analyzed by Western blot using specific antibodies to monitor the levels of huGwl, ERK, Plk1, huBora and phosphorylation of Plx1 on T201 (pPlk1), PP1 phosphatase on threonine 320 (pPP1), Cdk on Tyr15 (pTyr), and ERK on Thr202 and Tyr204. The green and red areas at bottom represent high and low CyclinB-Cdk1 (MPF) activity periods. ...View More
Tavernier, N., Thomas, Y., Vigneron, S., Maisonneuve, P., Orlicky, S., Mader, P., ... & Pintard, L. (2021). Bora phosphorylation substitutes in trans for T-loop phosphorylation in Aurora A to promote mitotic entry. Nature communications, 12(1), 1899.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-0726
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, E

Basic Information

Immunogen
Amino acids 1-187 mapping at the N-terminus of Bora of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
BORA aurora kinase A activator
Introduction
BORA is an activator of the protein kinase Aurora A (AURKA; MIM 603072), which is required for centrosome maturation, spindle assembly, and asymmetric protein localization during mitosis .
Entrez Gene ID
79866
UniProt ID
Q6PGQ7
Alternative Names
Bora, Aurora Kinase A Activator; C13orf34; Chromosome 13 Open Reading Frame 34; Protein Aurora Borealis; HsBora;
Function
Required for the activation of AURKA at the onset of mitosis.
Biological Process
Activation of protein kinase activity Source: GO_Central
Cell division Source: UniProtKB-KW
G2/M transition of mitotic cell cycle Source: Reactome
Regulation of mitotic nuclear division Source: UniProtKB
Regulation of mitotic spindle organization Source: UniProtKB
Regulation of protein localization Source: UniProtKB
Cellular Location
Cytosol; Nucleus; Cytoplasm
PTM
Phosphorylated by AURKA.
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For research use only. Not intended for any clinical use.

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