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Mouse Anti-BLZF1 Recombinant Antibody (CBYY-0630) (CBMAB-0633-YY)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYY-0630
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, E

Basic Information

Immunogen
Amino acids 1-300 mapping at the N-terminus of golgin 45 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Basic Leucine Zipper Nuclear Factor 1
Introduction
Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.
Entrez Gene ID
8548
UniProt ID
Q9H2G9
Alternative Names
Basic Leucine Zipper Nuclear Factor 1; P45 Basic Leucine-Zipper Nuclear Factor; JEM-1; JEM1; Cytoplasmic Protein; JEM-1short Protein; GOLGIN-45; JEM-1s;
Function
Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.
Biological Process
Golgi organization Source: MGI
Golgi to plasma membrane protein transport Source: MGI
Cellular Location
Golgi apparatus membrane
Isoform 1: Nucleus. Detected in the nucleus upon heterologous expression. Not detected in the cytoplasm.
Isoform 2: Cytoplasm. Not detected in the nucleus.
PTM
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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