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Mouse Anti-BIRC7 Recombinant Antibody (CBYJL-178) (CBMAB-L0256-YJ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJL-178
Antibody Isotype
IgG1
Application
WB, IHC, FC

Basic Information

Immunogen
Full length human recombinant protein of human Livin produced in HEK293T cell.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100
IF(ICC)1:50
FC1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
BIRC7
Introduction
BIRC7 is a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is very important for inhibitory activity and interacts with caspases, whereas the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and participate in chemotherapy sensitivity. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
79444
UniProt ID
Q96CA5
Alternative Names
Baculoviral IAP Repeat Containing 7; Melanoma Inhibitor Of Apoptosis Protein; Kidney Inhibitor Of Apoptosis Protein; Livin; RING-Type E3 Ubiquitin Transferase BIRC7; RING Finger Protein 50; ML-IAP; MLIAP
Function
Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. Isoform 2 protects against natural killer (NK) cell killing whereas isoform 1 augments killing.
Biological Process
Activation of JUN kinase activity Source: UniProtKB
Lens development in camera-type eye Source: Ensembl
Negative regulation of apoptotic process Source: UniProtKB
Positive regulation of protein ubiquitination Source: GO_Central
Protein ubiquitination Source: UniProtKB
Regulation of cell cycle Source: GO_Central
Regulation of cell population proliferation Source: UniProtKB
Regulation of natural killer cell apoptotic process Source: UniProtKB
Cellular Location
Golgi apparatus; Cytoplasm; Nucleus. Nuclear, and in a filamentous pattern throughout the cytoplasm. Full-length livin is detected exclusively in the cytoplasm, whereas the truncated form (tLivin) is found in the peri-nuclear region with marked localization to the Golgi apparatus; the accumulation of tLivin in the nucleus shows positive correlation with the increase in apoptosis.
PTM
Autoubiquitinated and undergoes proteasome-mediated degradation.
The truncated protein (tLivin) not only loses its anti-apoptotic effect but also acquires a pro-apoptotic effect.
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For research use only. Not intended for any clinical use.

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