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Mouse Anti-BirA Recombinant Antibody (6C4c7) (CBMAB-AP639LY)


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Published Data
Mouse Anti-BirA Recombinant Antibody (6C4c7) (CBMAB-AP639LY) in WB
U2OS-FT stable cell lines expressing myc-BirA*, myc-BirA*-DDB1, GFP, and GFP-DDB1 were treated during 24 h with doxycycline (10 μM) to induce the expression of fused proteins. Cells were lysed and protein expression was determined anti-BirA* and anti-GFP antibodies. ...View More
Raisch, J., Dubois, M. L., Groleau, M., Lévesque, D., Burger, T., Jurkovic, C. M., ... & Boisvert, F. M. (2023). Pulse-SILAC and Interactomics Reveal Distinct DDB1-CUL4-Associated Factors, Cellular Functions, and Protein Substrates. Molecular & Cellular Proteomics, 22(10).
Mouse Anti-BirA Recombinant Antibody (6C4c7) (CBMAB-AP639LY) in IF
Immunofluorescence using anti-BirA* antibody and autofluorescence of GFP in U2OS-FT stable cell lines expressing myc-BirA*, myc-BirA*-DDB1, GFP, and GFP-DDB1 treated during 24 h with doxycycline (10 μM). ...View More
Raisch, J., Dubois, M. L., Groleau, M., Lévesque, D., Burger, T., Jurkovic, C. M., ... & Boisvert, F. M. (2023). Pulse-SILAC and Interactomics Reveal Distinct DDB1-CUL4-Associated Factors, Cellular Functions, and Protein Substrates. Molecular & Cellular Proteomics, 22(10).
Mouse Anti-BirA Recombinant Antibody (6C4c7) (CBMAB-AP639LY) in WB
Immunoblot analysis of inducible SK-N-AS cells expressing BirA* (SK-N-AS.BirA*) or ALK-BirA* (SK-N-AS.ALK-BirA*) upon doxycycline addition, in the presence or absence of 50 µM biotin as indicated. ...View More
Uckun, E., Siaw, J. T., Guan, J., Anthonydhason, V., Fuchs, J., Wolfstetter, G., ... & Palmer, R. H. (2021). BioID-screening identifies PEAK1 and SHP2 as components of the ALK proximitome in neuroblastoma cells. Journal of Molecular Biology, 433(19), 167158.
Mouse Anti-BirA Recombinant Antibody (6C4c7) (CBMAB-AP639LY) in IF
SK-N-AS.BirA* and SK-N-AS.ALK-BirA* as well as SK-N-BE(2).BirA* and SK-N-BE(2).ALK-BirA* cells were stained for ALK (red, all panels), BirA* (green, upper panel), biotin (green, lower panel) and DAPI (blue, all panels). ...View More
Uckun, E., Siaw, J. T., Guan, J., Anthonydhason, V., Fuchs, J., Wolfstetter, G., ... & Palmer, R. H. (2021). BioID-screening identifies PEAK1 and SHP2 as components of the ALK proximitome in neuroblastoma cells. Journal of Molecular Biology, 433(19), 167158.

Summary

Host Animal
Mouse
Specificity
E.coli
Clone
6C4c7
Antibody Isotype
IgG1, κ
Application
IF, WB

Basic Information

Immunogen
Amino acids residues 2-150 R118G of E.coli BirA.
Host Species
Mouse
Specificity
E.coli
Antibody Isotype
IgG1, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)1:10-1:500
WB1:100-1-2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
BirA
Function
Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.
Biological Process
Biotin biosynthetic process Source: EcoliWiki
Biotin metabolic process Source: CAFA
Protein biotinylation Source: CACAO
Regulation of transcription, DNA-templated Source: UniProtKB-UniRule
Cellular Location
Cytoplasm; Transcription repressor complex
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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