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Mouse Anti-BDNF Recombinant Antibody (3D9C5) (CBMAB-0117CQ)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
3D9C5
Antibody Isotype
IgG1
Application
IF, WB, ELISA

Basic Information

Immunogen
Purified recombinant fragment of human BDNF (AA: 19-248) expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:10,000
WB1:500-1:2,000
IF(ICC)1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Brain Derived Neurotrophic Factor
Introduction
This gene encodes a member of the nerve growth factor family of proteins. Human BDNF, which encodes a deduced 247 preproprotein that is proteolytically processed into a mature 119-amino acid protein. The mature BDNF protein shares 100% identity with mature porcine Bdnf and 52% identity with mature human NGF. Northern blot analysis detected BDNF transcripts of 1.6 and 4.0 kb in all brain regions examined. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders.
Entrez Gene ID
627
UniProt ID
P23560
Alternative Names
Brain-Derived Neurotrophic Factor; Neurotrophin; Abrineurin; ANON2; BULN2
Function
Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678).
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
BDNF precursor form: Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2 (PubMed:24908487, PubMed:29909994).
Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity).
Biological Process
Activation of phospholipase C activity Source: Reactome
Axon guidance Source: BHF-UCL
Brain-derived neurotrophic factor receptor signaling pathway Source: BHF-UCL
Collateral sprouting Source: BHF-UCL
Memory Source: GO_Central
Modulation of chemical synaptic transmission Source: GO_Central
Negative regulation of apoptotic signaling pathway Source: ARUK-UCL
Negative regulation of myotube differentiation Source: ParkinsonsUK-UCL
Negative regulation of neuron apoptotic process Source: GO_Central
Nerve development Source: GO_Central
Nerve growth factor signaling pathway Source: GO_Central
Nervous system development Source: ProtInc
Neuron projection morphogenesis Source: GO_Central
Neurotrophin TRK receptor signaling pathway Source: Reactome
Peripheral nervous system development Source: GO_Central
Positive regulation of brain-derived neurotrophic factor receptor signaling pathway Source: BHF-UCL
Positive regulation of collateral sprouting Source: BHF-UCL
Positive regulation of neuron projection development Source: ARUK-UCL
Positive regulation of non-membrane spanning protein tyrosine kinase activity Source: Reactome
Positive regulation of peptidyl-serine phosphorylation Source: GO_Central
Positive regulation of receptor binding Source: ParkinsonsUK-UCL
Positive regulation of synapse assembly Source: BHF-UCL
Regulation of neuron differentiation Source: GO_Central
Regulation of protein localization to cell surface Source: ParkinsonsUK-UCL
Synapse assembly Source: BHF-UCL
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Cellular Location
Secreted
BDNF precursor form: Secreted. A proportion of BDNF is secreted as immature precursor (proBDNF).
Involvement in disease
Congenital central hypoventilation syndrome (CCHS): Rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
PTM
BDNF precursor form: N-glycosylated and glycosulfated, contrary to mature BDNF.
Mature BDNF is produced by proteolytic removal of the propeptide, catalyzed by a FURIN family member. In addition, the precursor form is proteolytically cleaved within the propeptide, but this is not an obligatory intermediate for the production of mature BDNF (PubMed:11152678). Can be converted into mature BDNF by plasmin (PLG) (PubMed:19467646).
More Infomation

Chow, R., Wessels, J. M., & Foster, W. G. (2020). Brain-derived neurotrophic factor (BDNF) expression and function in the mammalian reproductive Tract. Human reproduction update, 26(4), 545-564.

Miranda, M., Morici, J. F., Zanoni, M. B., & Bekinschtein, P. (2019). Brain-derived neurotrophic factor: a key molecule for memory in the healthy and the pathological brain. Frontiers in cellular neuroscience, 13, 363.

Gejl, A. K., Enevold, C., Bugge, A., Andersen, M. S., Nielsen, C. H., & Andersen, L. B. (2019). Associations between serum and plasma brain-derived neurotrophic factor and influence of storage time and centrifugation strategy. Scientific reports, 9(1), 1-9.

Ng, T. K. S., Ho, C. S. H., Tam, W. W. S., Kua, E. H., & Ho, R. C. M. (2019). Decreased serum brain-derived neurotrophic factor (BDNF) levels in patients with Alzheimer’s disease (AD): a systematic review and meta-analysis. International journal of molecular sciences, 20(2), 257.

El Hayek, L., Khalifeh, M., Zibara, V., Abi Assaad, R., Emmanuel, N., Karnib, N., ... & Sleiman, S. F. (2019). Lactate mediates the effects of exercise on learning and memory through SIRT1-dependent activation of hippocampal brain-derived neurotrophic factor (BDNF). Journal of Neuroscience, 39(13), 2369-2382.

Giacobbo, B. L., Doorduin, J., Klein, H. C., Dierckx, R. A., Bromberg, E., & de Vries, E. F. (2019). Brain-derived neurotrophic factor in brain disorders: focus on neuroinflammation. Molecular neurobiology, 56(5), 3295-3312.

Notaras, M., & van den Buuse, M. (2019). Brain-derived neurotrophic factor (BDNF): novel insights into regulation and genetic variation. The Neuroscientist, 25(5), 434-454.

Sandrini, L., Di Minno, A., Amadio, P., Ieraci, A., Tremoli, E., & Barbieri, S. S. (2018). Association between obesity and circulating brain-derived neurotrophic factor (BDNF) levels: systematic review of literature and meta-analysis. International journal of molecular sciences, 19(8), 2281.

Hung, C. L., Tseng, J. W., Chao, H. H., Hung, T. M., & Wang, H. S. (2018). Effect of acute exercise mode on serum brain-derived neurotrophic factor (BDNF) and task switching performance. Journal of clinical medicine, 7(10), 301.

Lu, Y., Ho, C. S., McIntyre, R. S., Wang, W., & Ho, R. C. (2018). Agomelatine-induced modulation of brain-derived neurotrophic factor (BDNF) in the rat hippocampus. Life sciences, 210, 177-184.

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For research use only. Not intended for any clinical use.

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