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Rat Anti-BCAP31 Recombinant Antibody (CBYY-0273) (CBMAB-0274-YY)


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Published Data
Rat Anti-BCAP31 Recombinant Antibody (CBYY-0273) (CBMAB-0274-YY) in IF
Colocalization of BAP31 and CD11b/CD18 in adherent and spread PMN. PMN adherent to glass slides were fixed in 3.7% paraformaldehyde and permeabilized with 0.1% Triton X-100 followed by labeling with mAbs E1B2 (green) and LM2/1 (red). Note that BAP31 and CD11b colocalize in PMN granular structures (arrowheads). Bar, 10 μm. ...View More
Zen, K., Utech, M., Liu, Y., Soto, I., Nusrat, A., & Parkos, C. A. (2004). Association of BAP31 with CD11b/CD18: potential role in intracellular trafficking of CD11b/CD18 in neutrophils. Journal of Biological Chemistry, 279(43), 44924-44930.
Rat Anti-BCAP31 Recombinant Antibody (CBYY-0273) (CBMAB-0274-YY) in WB
Western blot analysis of (A) Aminopeptidase N (CD13), (B) Integrin α-2 (CD49b) and (C) VDAC-1 after treatment of HUVECs with OxPAPC. Samples were treated for 0 or 90 min with a final OxPAPC concentration of 0 or 80 µmol/L. BAP31: B-cell receptor-associated protein 31 which was identified to not be modified by OxPAPC. ...View More
Gesslbauer, B., Kuerzl, D., Valpatic, N., & Bochkov, V. N. (2018). Unbiased identification of proteins covalently modified by complex mixtures of peroxidized lipids using a combination of electrophoretic mobility band shift with mass spectrometry. Antioxidants, 7(9), 116.

Summary

Host Animal
Rat
Specificity
Human, Cattle, Hamster
Clone
CBYY-0273
Antibody Isotype
IgG2a
Application
WB, FC, ICC, IHC-P, IP

Basic Information

Immunogen
Amino acid residues 123-229 of human BAP31.
Host Species
Rat
Specificity
Human, Cattle, Hamster
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,500
IHC-P1:200-1:6,400
FC1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Ascites
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
B Cell Receptor Associated Protein 31
Introduction
This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. [provided by RefSeq, Jan 2012]
Entrez Gene ID
Human10134
Cattle533949
UniProt ID
HumanP51572
CattleQ5E9F1
Alternative Names
B Cell Receptor Associated Protein 31; C6-AG Tumor-Associated Antigen; DXS1357E; BAP31; B-Cell Receptor-Associated Protein 31; B-Cell Receptor Associated Protein 31; BCR-Associated Protein Bap31; BCR-Associated Protein 31;
Function
Functions as a chaperone protein (PubMed:9396746, PubMed:18287538).
Is one of the most abundant endoplasmic reticulum (ER) proteins (PubMed:9396746, PubMed:18287538).
Plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD) (PubMed:9396746, PubMed:18287538).
Also serves as a cargo receptor for the export of transmembrane proteins (By similarity).
Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by stimulating the translocation of NDUFS4 and NDUFB11 from the cytosol to the mitochondria via interaction with TOMM40 (PubMed:31206022).
In response to ER stress, delocalizes from the ER-mitochondria contact sites and binds BCL2 (PubMed:31206022).
May be involved in CASP8-mediated apoptosis (PubMed:10958671).
Biological Process
Antigen processing and presentation of peptide antigen via MHC class I Source: Reactome
Apoptotic process Source: UniProtKB-KW
Calcium-mediated signaling using intracellular calcium source Source: UniProtKB
Endoplasmic reticulum to Golgi vesicle-mediated transport Source: GO_Central
Negative regulation of endoplasmic reticulum calcium ion concentration Source: UniProtKB
Positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Positive regulation of cytosolic calcium ion concentration Source: UniProtKB
Positive regulation of ER-associated ubiquitin-dependent protein catabolic process Source: ParkinsonsUK-UCL
Positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
Positive regulation of mitochondrial calcium ion concentration Source: UniProtKB
Positive regulation of retrograde protein transport, ER to cytosol Source: ParkinsonsUK-UCL
Protein localization to endoplasmic reticulum exit site Source: GO_Central
Protein targeting to mitochondrion Source: UniProtKB
Response to endoplasmic reticulum stress Source: UniProtKB
Spermatogenesis Source: Ensembl
Viral process Source: UniProtKB-KW
Cellular Location
Endoplasmic reticulum membrane; Endoplasmic reticulum-Golgi intermediate compartment membrane. May shuttle between the ER and the intermediate compartment/cis-Golgi complex (PubMed:9396746). Associates with the mitochondria-associated endoplasmic reticulum membrane via interaction with TOMM40 (PubMed:31206022).
Involvement in disease
Deafness, dystonia, and cerebral hypomyelination (DDCH): An X-linked recessive mental retardation syndrome characterized by almost no psychomotor development, dysmorphic facial features, sensorineural deafness, dystonia, pyramidal signs, and hypomyelination on brain imaging.
Topology
Lumenal: 2-6 aa
Helical: 7-27 aa
Cytoplasmic: 28-43 aa
Helical: 44-64 aa
Lumenal: 65-102 aa
Helical: 103-123 aa
Cytoplasmic: 124-246 aa
PTM
Cleaved by CASP8 and other caspases.
More Infomation

Liang, H., Dong, J., Cheng, Z., Li, Q., Feng, D., & Ling, B. (2021). B‑cell receptor‑associated protein 31 promotes migration and invasion in ovarian cancer cells. Experimental and Therapeutic Medicine, 22(2), 1-12.

Liu, T., Yu, J., Ge, C., Zhao, F., Miao, C., Jin, W., ... & Li, H. (2021). B-cell receptor-associated protein 31 promotes metastasis via AKT/β-catenin/Snail pathway in hepatocellular carcinoma. Frontiers in molecular biosciences, 8.

Namusamba, M., Li, Z., Zhang, Q., Wang, C., Wang, T., & Wang, B. (2021). Biological roles of the B cell receptor-associated protein 31: Functional Implication in Cancer. Molecular Biology Reports, 1-14.

Abdou, A. G., Diab, A., & Marae, A. (2020). Immunohistochemical expression of BCAP 31 in chronic plaque psoriasis. Journal of Immunoassay and Immunochemistry, 41(5), 852-863.

Wang, J., Jiang, D., Li, Z., Yang, S., Zhou, J., Zhang, G., ... & Yang, K. (2020). BCAP31, a cancer/testis antigen-like protein, can act as a probe for non-small-cell lung cancer metastasis. Scientific reports, 10(1), 1-14.

Jia, C. C., Li, G., Jiang, R., Liu, X., Yuan, Q., Le, W., ... & Wang, B. (2020). B-Cell Receptor-Associated Protein 31 Negatively Regulates the Expression of Monoamine Oxidase A Via R1. Frontiers in Molecular Biosciences, 7, 64.

Nagło, O. (2019). Functional characterization of B-cell receptor associated protein 31 in cancer cells (Doctoral dissertation, lmu).

Fu, W., Sun, H., Zhao, Y., Chen, M., Yang, X., Liu, Y., & Jin, W. (2019). BCAP31 drives TNBC development by modulating ligand-independent EGFR trafficking and spontaneous EGFR phosphorylation. Theranostics, 9(22), 6468.

Jia, C. C., Du, J., Liu, X., Jiang, R., Huang, Y., Wang, T., ... & Wang, B. (2018). B-cell receptor-associated protein 31 regulates the expression of valosin-containing protein through Elf2. Cellular Physiology and Biochemistry, 51(4), 1799-1814.

Ruchusatsawat, K., Thiemsing, L., Mutirangura, A., & Wongpiyabovorn, J. (2017). BCAP 31 expression and promoter demethylation in psoriasis. Asian Pacific journal of allergy and immunology, 35(2), 86-90.

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For research use only. Not intended for any clinical use.

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