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Mouse Anti-ATP6V1F Recombinant Antibody (1B8) (CBMAB-V0023-YC)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
1B8
Antibody Isotype
IgG2a
Application
WB, FC, IHC, ICC, IF

Basic Information

Immunogen
Full length human recombinant protein of human ATP6V1F produced in E.coli
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:2,000
IF(ICC)1:100
IHC1:150
FC1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 1% BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATPase H+ Transporting V1 Subunit F
Introduction
ATP6V1F is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. ATP6V1F is the V1 domain F subunit protein. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.
Entrez Gene ID
9296
UniProt ID
Q16864
Alternative Names
ATPase H+ Transporting V1 Subunit F; ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F; Vacuolar Proton Pump Subunit F; V-ATPase 14 KDa Subunit; V-ATPase Subunit F; ATP6S14; VATF; H(+)-Transporting Two-Sector ATPase, 14kD Subunit
Function
Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Biological Process
Cellular response to amino acid starvation Source: Reactome
Insulin receptor signaling pathway Source: Reactome
Ion transmembrane transport Source: Reactome
Phagosome acidification Source: Reactome
Proton transmembrane transport Source: UniProtKB
Transferrin transport Source: Reactome
Cellular Location
Cytosol; Extracellular exosome; Membrane; Proton-transporting two-sector ATPase complex; Proton-transporting V-type ATPase, V1 domain; Vacuolar proton-transporting V-type ATPase complex
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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