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Mouse Anti-ATP10A Recombinant Antibody (G-9) (CBMAB-A3999-YC)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
G-9
Antibody Isotype
IgG2b, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1001-1064 mapping near the C-terminus of ATP10A of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATPase Phospholipid Transporting 10A (Putative)
Introduction
ATP10A belongs to the family of P-type cation transport ATPases, and to the subfamily of aminophospholipid-transporting ATPases. The aminophospholipid translocases transport phosphatidylserine and phosphatidylethanolamine from one side of a bilayer to ano
Entrez Gene ID
Human57194
Mouse11982
Rat365266
UniProt ID
HumanO60312
MouseO54827
RatA0A0G2JWQ8
Alternative Names
ATPase Phospholipid Transporting 10A (Putative); ATP10C; P4-ATPase Flippase Complex Alpha Subunit ATP10A; Aminophospholipid Translocase VA; EC 3.6.3.1; ATPVA; ATPVC; ATPase Type IV, Phospholipid Transporting (P-Type);
Function
Catalytic component of P4-ATPase flippase complex, which catalyzes the hydrolysis of ATP coupled to the transport of phosphatidylcholine (PC) from the outer to the inner leaflet of the plasma membrane (PubMed:25947375, PubMed:29599178, PubMed:30530492).
Initiates inward plasma membrane bending and recruitment of Bin/amphiphysin/Rvs (BAR) domain-containing proteins involved in membrane tubulation and cell trafficking (PubMed:29599178).
Facilitates ITGB1/beta1 integrin endocytosis, delaying cell adhesion and cell spreading on extracellular matrix (PubMed:29599178, PubMed:25947375).
Has low flippase activity toward glucosylceramide (GlcCer) (PubMed:30530492).
Biological Process
Ion transmembrane transport Source: Reactome
Phospholipid translocation Source: GO_Central
Positive regulation of membrane tubulation Source: UniProtKB
Regulation of cell shape Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane; Cell membrane. Exit from the endoplasmic reticulum requires the presence of TMEM30A, but not that of TMEM30B.
Topology
Cytoplasmic: 1-86 aa
Helical: 87-106 aa
Exoplasmic loop: 107-110 aa
Helical: 111-128 aa
Cytoplasmic: 129-309 aa
Helical: 310-332 aa
Exoplasmic loop: 333-362 aa
Helical: 363-384 aa
Cytoplasmic: 385-1087 aa
Helical: 1088-1108 aa
Exoplasmic loop: 1109-1119 aa
Helical: 1120-1140 aa
Cytoplasmic: 1141-1170 aa
Helical: 1171-1192 aa
Exoplasmic loop: 1193-1199 aa
Helical: 1200-1222 aa
Cytoplasmic: 1223-1228 aa
Helical: 1229-1249 aa
Exoplasmic loop: 1250-1267 aa
Helical: 1268-1292 aa
Cytoplasmic: 1293-1499 aa
PTM
Autophosphorylated at the conserved aspartate of the P-type ATPase signature sequence.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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