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Rabbit Anti-ATF7 Recombinant Antibody (EG289) (CBMAB-EN318-LY)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
EG289
Antibody Isotype
IgG
Application
FC, IF, IHC, WB

Basic Information

Immunogen
Synthetic peptide within Human ATF7 aa 1-50.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:1,000
FC1:50-1:100
IHC1:50-1:200
IF(ICC)1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
TBS, pH 7.4, 40% Glycerol, 0.05% BSA
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Activating Transcription Factor 7
Introduction
Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5-GTGACGT[AG][AG]-3), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family.
Entrez Gene ID
Human11016
Mouse223922
UniProt ID
HumanP17544
MouseQ8R0S1
Alternative Names
Activating Transcription Factor 7; Transcription Factor ATF-A; ATFA; Cyclic AMP-Dependent Transcription Factor ATF-7; CAMP-Dependent Transcription Factor ATF-7;
Function
Stress-responsive chromatin regulator that plays a role in various biological processes including innate immunological memory, adipocyte differentiation or telomerase regulation (PubMed:29490055).
In absence of stress, contributes to the formation of heterochromatin and heterochromatin-like structure by recruiting histone H3K9 tri- and di-methyltransferases thus silencing the transcription of target genes such as STAT1 in adipocytes, or genes involved in innate immunity in macrophages and adipocytes (By similarity).
Stress induces ATF7 phosphorylation that disrupts interactions with histone methyltransferase and enhances the association with coactivators containing histone acetyltransferase and/or histone demethylase, leading to disruption of the heterochromatin-like structure and subsequently transcriptional activation (By similarity).
In response to TNF-alpha, which is induced by various stresses, phosphorylated ATF7 and telomerase are released from telomeres leading to telomere shortening (PubMed:29490055).
Plays also a role in maintaining epithelial regenerative capacity and protecting against cell death during intestinal epithelial damage and repair (By similarity).
Isoform 4: Acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter.
Isoform 5: Acts as a negative regulator, inhibiting both ATF2 and ATF7 transcriptional activities. It may exert these effects by sequestrating in the cytoplasm the Thr-53 phosphorylating kinase, preventing activation.
Biological Process
Negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Viral process Source: UniProtKB-KW
Cellular Location
Nucleus; Nucleoplasm; Telomere. Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear periphery.
Isoform 5: Cytoplasm
PTM
On EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity.
Sumoylation delays nuclear localization and inhibits transactivation activity through preventing binding to TAF12. RANBP2 appears to be the specific E3 ligase.
On EGF stimulation, phosphorylated first on Thr-53 allowing subsequent phosphorylation on Thr-51. This latter phosphorylation prevents sumoylation, increases binding to TAF12 and enhances transcriptional activity (PubMed:18950637). Social isolation stress as well as TNF-alpha also induce the phosphorylation of ATF7 (PubMed:29490055). Phosphorylated in proliferating colonic and small intestinal epithelial cells (By similarity).
More Infomation

Chen, M., Liu, Y., Yang, Y., Qiu, Y., Wang, Z., Li, X., & Zhang, W. (2021). Emerging roles of activating transcription factor (ATF) family members in tumourigenesis and immunity: Implications in cancer immunotherapy. Genes & Diseases.

Wimberly, K., & Choe, K. (2019). The extracellular matrix signals through protein kinase MEKK‐3 and transcription factor ATF‐7 to activate cytoprotective genes. The FASEB Journal, 33(S1), 719-16.

Song, F., Wei, M., Wang, J., Liu, Y., Guo, M., Li, X., ... & Sun, G. (2019). Hepatitis B virus‐regulated growth of liver cancer cells occurs through the microRNA‐340‐5p‐activating transcription factor 7‐heat shock protein A member 1B axis. Cancer science, 110(5), 1633-1643.

Liu, Y., Maekawa, T., Yoshida, K., Muratani, M., Chatton, B., & Ishii, S. (2019). The transcription factor ATF7 controls adipocyte differentiation and thermogenic gene programming. IScience, 13, 98-112.

Fletcher, M., Tillman, E. J., Butty, V. L., Levine, S. S., & Kim, D. H. (2019). Global transcriptional regulation of innate immunity by ATF-7 in C. elegans. PLoS genetics, 15(2), e1007830.

Liu, Y., Maekawa, T., Yoshida, K., Kaneda, H., Chatton, B., Wakana, S., & Ishii, S. (2017). The transcription factor ATF 7 mediates in vitro fertilization‐induced gene expression changes in mouse liver. FEBS open bio, 7(10), 1598-1610.

Hall, J. A., McElwee, M. K., & Freedman, J. H. (2017). Identification of ATF-7 and the insulin signaling pathway in the regulation of metallothionein in C. elegans suggests roles in aging and reactive oxygen species. PloS one, 12(6), e0177432.

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For research use only. Not intended for any clinical use.

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