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Mouse Anti-ARHGAP5 Recombinant Antibody (54/P190-B) (CBMAB-P0070-YC)


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Published Data
Mouse Anti-ARHGAP5 Recombinant Antibody (54/P190-B) CBMAB-P0070-YC in IF
Immunofluorescence images of fixed RAW 264.7 macrophages challenged with C3biopsonized beads for 45 minutes and stained with phalloidin for F-actin and antibodies to RhoA, p190RhoGAP, p34 Arp2/3 or WASP. Arrows indicate F-actin flashing phagosomes, while arrowheads indicate non-flashing phagosomes. ...View More
Poirier, M. B., Fiorino, C., Rajasekar, T. K., & Harrison, R. E. (2020). F-actin flashes on phagosomes mechanically deform contents for efficient digestion in macrophages. Journal of cell science, 133(12), jcs239384.
Mouse Anti-ARHGAP5 Recombinant Antibody (54/P190-B) CBMAB-P0070-YC in WB
The ARHGAP5 expression in HTR8/SVneo cells was analyzed by Western blot analysis at the mRNA and protein levels, respectively.
Taga, S., Hayashi, M., Nunode, M., Nakamura, N., & Ohmichi, M. (2022). miR-486-5p inhibits invasion and migration of HTR8/SVneo trophoblast cells by down-regulating ARHGAP5. Placenta, 123, 5-11.
Mouse Anti-ARHGAP5 Recombinant Antibody (54/P190-B) CBMAB-P0070-YC in IP
Co-IP assay detected binding of both RhoE and RhoEα to Syx and p190RhoGAP. HEK293 cells were transfected with indicated plasmids. Twenty four hours after transfection, cell lysates were used for Co-IP assay with anti-flag antibody. ...View More
Dai, Y., Luo, W., Yue, X., Ma, W., Wang, J., & Chang, J. (2020). Identification and characterization of a new isoform of small GTPase RhoE. Communications Biology, 3(1), 572.
Mouse Anti-ARHGAP5 Recombinant Antibody (54/P190-B) CBMAB-P0070-YC in IF
Immunofluorescence images of fixed RAW 264.7 macrophages challenged with C3biopsonized beads for 45 minutes and stained with phalloidin for F-actin and antibodies to RhoA, p190RhoGAP, p34 Arp2/3 or WASP. Arrows indicate F-actin flashing phagosomes, while arrowheads indicate non-flashing phagosomes. ...View More
Poirier, M. B., Fiorino, C., Rajasekar, T. K., & Harrison, R. E. (2020). F-actin flashes on phagosomes mechanically deform contents for efficient digestion in macrophages. Journal of cell science, 133(12), jcs239384.
Mouse Anti-ARHGAP5 Recombinant Antibody (54/P190-B) CBMAB-P0070-YC in WB
The ARHGAP5 expression in HTR8/SVneo cells was analyzed by Western blot analysis at the mRNA and protein levels, respectively.
Taga, S., Hayashi, M., Nunode, M., Nakamura, N., & Ohmichi, M. (2022). miR-486-5p inhibits invasion and migration of HTR8/SVneo trophoblast cells by down-regulating ARHGAP5. Placenta, 123, 5-11.

Summary

Host Animal
Mouse
Specificity
Human
Clone
54/P190-B
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1091-1250 mapping within an internal region of Rho GAP p190-B of human origin.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Rho GTPase Activating Protein 5
Introduction
Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP.
Entrez Gene ID
Human23092
Dog490642
Rat307459
UniProt ID
HumanQ9UNA1
DogA0A5F4CSE9
RatQ9ERC1
Alternative Names
GFI2; RhoGAP5; p190-B; p190BRhoGAP
Function
GTPase-activating protein for Rho family members (PubMed:8537347).
Biological Process
Cell adhesion Source: ProtInc
Mammary gland development Source: Ensembl
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: ProtInc
Cellular Location
Cell membrane; Cytoplasm. Also membrane-associated in a fibrillar pattern that colocalizes with the alpha5-beta1 integrin receptor (ITGA5/ITGB1) for fibronectin.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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