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Mouse Anti-APBB1 Recombinant Antibody (D11) (CBMAB-A3016-YC)


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Published Data
Mouse Anti-APBB1 Recombinant Antibody (D11) in IF
Immunofluorescence for MAP1B (white), Fe65 (red), APP (green), and DAPI for nuclei staining (blue) in mice hippocampal cells in control and treatment with Aβo (0.5 μM, 24 h). ...View More
Godoy, P. A., Mennickent, D., Cuchillo-Ibanez, I., Ramírez-Molina, O., Silva-Grecchi, T., Panes-Fernández, J., ... & Fuentealba, J. (2021). Increased P2× 2 receptors induced by amyloid-β peptide participates in the neurotoxicity in alzheimer's disease. Biomedicine & Pharmacotherapy, 142, 111968.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
D11
Antibody Isotype
IgG3, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 63-101 near the N-terminus of Fe65 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG3, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 254-293

Target

Full Name
Amyloid Beta Precursor Protein Binding Family B Member 1
Introduction
APBB1 is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related
Entrez Gene ID
11785
UniProt ID
Q9QXJ1
Alternative Names
Amyloid Beta Precursor Protein Binding Family B Member 1; Amyloid Beta (A4) Precursor Protein-Binding, Family B, Member 1 (Fe65); FE65; RIR; Amyloid Beta A4 Precursor Protein-Binding Family B Member 1; Amyloid-Beta A4 Precursor Protein-Binding Family B Me
Function
Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity. Functions in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).
Biological Process
Apoptotic process Source: UniProtKB-KW
Axonogenesis Source: UniProtKB
Cell cycle arrest Source: UniProtKB
Cellular response to DNA damage stimulus Source: UniProtKB
Double-strand break repair Source: Reactome
Histone H4 acetylation Source: UniProtKB
Negative regulation of cell growth Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: ARUK-UCL
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of protein secretion Source: ARUK-UCL
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: ARUK-UCL
Regulation of transcription, DNA-templated Source: GO_Central
Signal transduction Source: UniProtKB
Cellular Location
Cytoplasm; Cell membrane; Nucleus; Nucleus speckle; Growth cone. Colocalizes with TSHZ3 in axonal growth cone (By similarity). In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via its interaction with APP. Following exposure to DNA damaging agents, it is released from cell membrane and translocates to the nucleus. Nuclear translocation is under the regulation of APP. Colocalizes with TSHZ3 in the nucleus. Colocalizes with NEK6 at the nuclear speckles. Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus (By similarity).
PTM
Polyubiquitination by RNF157 leads to degradation by the proteasome (PubMed:25342469).
Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus (By similarity). Phosphorylated following nuclear translocation. Phosphorylation at Tyr-547 by ABL1 enhances transcriptional activation activity and reduces the affinity for RASD1/DEXRAS1.
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For research use only. Not intended for any clinical use.

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