Rabbit Anti-AGXT Recombinant Antibody (V2-180259) (CBMAB-A1692-YC)

Name: Rabbit Anti-AGXT Recombinant Antibody (V2-180259)
SKU: CBMAB-A1692-YC
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-AGXT Recombinant Antibody (CBYC-A437) (CBMAB-A1692-YC) in Immunoblot

Immunoblot of Agxt in fasted and refed mutant (KO, n = 3) and control (WT, n = 3) mice. Representative blot of two independent experiments. ...View More

Just, P. A., Charawi, S., Denis, R. G., Savall, M., Traore, M., Foretz, M., ... & Perret, C. (2020). Lkb1 suppresses amino acid-driven gluconeogenesis in the liver. Nature Communications, 11(1), 6127.

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse

Clone

V2-180259

Antibody Isotype

IgG

Application

WB, IP, IHC-P

Basic Information

Immunogen

Recombinant fragment corresponding to Human AGXT.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000-1:10,000
IP	1:10-1:100
IHC-P	1:100-1:250

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, 40% Glycerol, 0.05% BSA

Preservative

0.01% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Alanine-Glyoxylate Aminotransferase

Introduction

AGXT is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I pri

Entrez Gene ID

Human	189
Mouse	11611

UniProt ID

Human	P21549
Mouse	O35423

Alternative Names

Alanine-Glyoxylate Aminotransferase; L-Alanine: Glyoxylate Aminotransferase 1; SPAT; AGT1; AGT; SPT; Hepatic Peroxisomal Alanine:Glyoxylate Aminotransferase; Alanine--Glyoxylate Aminotransferase; Serine--Pyruvate Aminotransferase; Serine:Pyruvate Aminotra

Biological Process

Carboxylic acid metabolic process
Glycine biosynthetic process, by transamination of glyoxylate
Glyoxylate catabolic process
Glyoxylate metabolic process
L-alanine catabolic process
L-cysteine catabolic process
Protein localization
Pyruvate biosynthetic process
Response to cAMP
Response to glucocorticoid

Cellular Location

Peroxisome; Mitochondrion. Predominantly localized in the peroxisomes. Mitochondrial mistargeting occurs in variant proteins Arg-41, Arg-47, Ile-152, Arg-170 and Thr-244 associated with the disease HP1.

Involvement in disease

Hyperoxaluria primary 1 (HP1): An inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and progressive tissue accumulation of insoluble calcium oxalate. Affected individuals are at risk for nephrolithiasis, nephrocalcinosis and early onset end-stage renal disease.

More Infomation

References

Li, Y., Zheng, R., Xu, G., Huang, Y., Li, Y., Li, D., & Geng, H. (2021). Generation and characterization of a novel rat model of primary hyperoxaluria type 1 with a nonsense mutation in alanine-glyoxylate aminotransferase gene. American Journal of Physiology-Renal Physiology, 320(3), F475-F484.

Wang, W., Li, J., Ning, C., & Tian, Y. (2020). Mutational analysis of AGXT gene in Chinese patients with primary hyperoxaluria type 1. Journal of Pediatric Urology, 16, S48-S49.

Sun, Y., Li, W., Shen, S., Yang, X., Lu, B., Zhang, X., ... & Ji, J. (2019). Loss of alanine-glyoxylate and serine-pyruvate aminotransferase expression accelerated the progression of hepatocellular carcinoma and predicted poor prognosis. Journal of translational medicine, 17(1), 1-16.

Lu, X., Chen, W., Li, L., Zhu, X., Huang, C., Liu, S., ... & Zhao, Y. (2019). Two novel AGXT mutations cause the infantile form of primary hyperoxaluria type I in a Chinese family: Research on missed mutation. Frontiers in pharmacology, 10, 85.

Li, X., Gu, J., Yang, Y., Li, J., & Li, Y. (2018). A putative mutation hotspot of the AGXT gene associated with primary Hyperoxaluria type 1 in the Chinese population. The Tohoku journal of experimental medicine, 246(4), 233-241.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

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Mouse Anti-AGXT Recombinant Antibody (V2-180256)

Mouse Anti-AGXT Recombinant Antibody (1F9BC7)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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