Mouse Anti-ACOT12 Recombinant Antibody (V2-179342) (CBMAB-A0642-YC)

Name: Mouse Anti-ACOT12 Recombinant Antibody (V2-179342)
SKU: CBMAB-A0642-YC
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-179342

Antibody Isotype

IgG2a

Application

FC, WB

Basic Information

Immunogen

Full length recombinant protein of human ACOT112 (NP_570123) produced in HEK293T cell.

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:2,000
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Buffer

PBS, pH7.3, 1% BSA, 50% Glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Acyl-CoA Thioesterase 12

Introduction

ACOT12 hydrolyzes acetyl-CoA to acetate and CoA.

Entrez Gene ID

134526

UniProt ID

Q8WYK0

Alternative Names

Acyl-CoA Thioesterase 12; StAR-Related Lipid Transfer (START) Domain Containing 15; Cytoplasmic Acetyl-CoA Hydrolase 1; START Domain-Containing Protein 15; Acyl-CoA Thioester Hydrolase 12; EC 3.1.2.1; STARD15; HCACH-1;

Function

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Acyl-coenzyme A thioesterase 12/ACOT12 preferentially hydrolyzes acetyl-CoA.

Biological Process

Acetyl-CoA metabolic process
Acyl-CoA metabolic process
Fatty acid metabolic process

Cellular Location

Cytosol

More Infomation

References

Lu, M., Zhu, W. W., Wang, X., Tang, J. J., Zhang, K. L., Yu, G. Y., ... & Qin, L. X. (2019). ACOT12-dependent alteration of acetyl-CoA drives hepatocellular carcinoma metastasis by epigenetic induction of epithelial-mesenchymal transition. Cell metabolism, 29(4), 886-900.

Acuna‐Aravena, M., & Cohen, D. E. (2018). Regulation of Hepatic Glucose and Lipid Metabolism by the Acetyl‐CoA Hydrolase Acyl‐CoA Thioesterase 12 (Acot12). The FASEB Journal, 32, 670-45.

Khandokar, Y. B., Srivastava, P., Cowieson, N., Sarker, S., Aragao, D., Das, S., ... & Forwood, J. K. (2017). Structural insights into GDP-mediated regulation of a bacterial acyl-CoA thioesterase. Journal of Biological Chemistry, 292(50), 20461-20471.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-ACOT12 Recombinant Antibody (V2-179341)

Mouse Anti-ACOT12 Recombinant Antibody (V2-179340)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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