Mouse Anti-ACIN1 Recombinant Antibody (V2-179307) (CBMAB-A0601-YC)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-179307

Antibody Isotype

IgG1

Application

ELISA, FC, ICC, WB

Basic Information

Immunogen

Partial sequence of recombinant full-length protein to human Acinus

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide

Buffer

PBS, pH7.4, 1% BSA, 50% Glycerol

Preservative

0.05% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Apoptotic Chromatin Condensation Inducer 1

Introduction

Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. This gene encodes a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA

Entrez Gene ID

22985

UniProt ID

Q9UKV3

Alternative Names

Apoptotic Chromatin Condensation Inducer 1; Apoptotic Chromatin Condensation Inducer In The Nucleus; Functional Spliceosome-Associated Protein 152; ACINUS; KIAA0670; FSAP152; ACN;

Function

Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells.

Biological Process

Apoptotic chromosome condensation
Erythrocyte differentiation
mRNA processing
Negative regulation of mRNA splicing, via spliceosome
Positive regulation of apoptotic process
Positive regulation of monocyte differentiation
RNA splicing

Cellular Location

Nucleus; Nucleus speckle; Nucleoplasm. Phosphorylation on Ser-1180 by SRPK2 redistributes it from the nuclear speckles to the nucleoplasm.

PTM

Phosphorylation on Ser-1180 by SRPK2 up-regulates its stimulatory effect on cyclin A1.
Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.

More Infomation

References

Chen, Y. S., Liu, C. W., Lin, Y. C., Tsai, C. Y., Yang, C. H., & Lin, J. C. (2020). The SRSF3-MBNL1-Acin1 circuit constitutes an emerging axis to lessen DNA fragmentation in colorectal cancer via an alternative splicing mechanism. Neoplasia, 22(12), 702-713.

Lin, Y. C., Lu, Y. H., Lee, Y. C., Hung, C. S., & Lin, J. C. (2020). Altered expressions and splicing profiles of Acin1 transcripts differentially modulate brown adipogenesis through an alternative splicing mechanism. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1863(9), 194601.

Pincez, T., Landry, J. R., Roussy, M., Jouan, L., Bilodeau, M., Laramée, L., ... & Cellot, S. (2020). Cryptic recurrent ACIN1‐NUTM1 fusions in non‐KMT2A‐rearranged infant acute lymphoblastic leukemia. Genes, Chromosomes and Cancer, 59(2), 125-130.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-ACIN1 Recombinant Antibody (V2-179305)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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