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Rabbit Anti-ACAT2 Recombinant Antibody (V2-179235) (CBMAB-A0496-YC)


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Published Data
Rabbit Anti-ACAT2 Recombinant Antibody (E1L8V) (CBMAB-A0496-YC) in WB
Alidation of systems analyses in predicting pHi-sensitive metabolic vulnerabilities. Efficiency of knockdown of indicated targets at the mRNA and protein levels, following reverse transfection of MCF7 cells with the indicated siRNAs. ...View More
Persi, E., Duran-Frigola, M., Damaghi, M., Roush, W. R., Aloy, P., Cleveland, J. L., ... & Ruppin, E. (2018). Systems analysis of intracellular pH vulnerabilities for cancer therapy. Nature communications, 9(1), 2997.

Summary

Host Animal
Rabbit
Specificity
Human, Monkey
Clone
V2-179235
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding Val216 of human ACAT2 protein.
Specificity
Human, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Acetyl-CoA Acetyltransferase 2
Introduction
ACAT2 is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of
Entrez Gene ID
Human39
Monkey741276
UniProt ID
HumanO75908
MonkeyO77759
Alternative Names
Acetyl-CoA Acetyltransferase 2; EC 2.3.1.9; Acetyl-Coenzyme A Acetyltransferase 2 (Acetoacetyl Coenzyme A Thiolase); Acetyl-Coenzyme A Acetyltransferase 2; Acetyl-CoA Transferase-Like Protein; Cytosolic Acetoacetyl-CoA Thiolase; Acetoacetyl Coenzyme A Thi
Function
Involved in the biosynthetic pathway of cholesterol.
Biological Process
Cholesterol biosynthetic process
Fatty acid beta-oxidation
Lipid metabolic process
Cellular Location
Cytosol
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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