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Mouse Anti-ABCB1 Recombinant Antibody (V2-178954) (CBMAB-A0189-YC)


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Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-178954
Antibody Isotype
IgG2a
Application
IHC-P, WB

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 995-1280 of human ABCB1 (NP_000918) produced in SF9 cell.
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IHC1:150

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Buffer
PBS, pH7.3, 1% BSA, 50% Glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATP Binding Cassette Subfamily B Member 1
Introduction
ABCB1 belongs to the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20,
Entrez Gene ID
5243
UniProt ID
P08183
Alternative Names
ATP Binding Cassette Subfamily B Member 1; ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1; Multidrug Resistance Protein 1; Colchicin Sensitivity; P-Glycoprotein 1; EC 3.6.3.44; MDR1; PGY1; ATP-Binding Cassette Sub-Family B Member 1; Doxorubicin Re
Function
Translocates drugs and phospholipids across the membrane. Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins. Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Biological Process
Carboxylic acid transmembrane transport
Ceramide translocation
Export across plasma membrane
G2/M transition of mitotic cell cycle
Phospholipid translocation
Positive regulation of anion channel activity
Regulation of chloride transport
Regulation of response to osmotic stress
Response to drug
Stem cell proliferation
Terpenoid transport
Transepithelial transport
Transmembrane transport
Transport across blood-brain barrier
Xenobiotic transport across blood-brain barrier
Cellular Location
Cell membrane; Apical cell membrane
Involvement in disease
A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Topology
Cytoplasmic: 1-44 aa
Helical: 45-67 aa
Extracellular: 68-116 aa
Helical: 117-137 aa
Cytoplasmic: 138-186 aa
Helical: 187-208 aa
Extracellular: 209-215 aa
Helical: 216-236 aa
Cytoplasmic: 237-292 aa
Helical: 293-316 aa
Extracellular: 317-330 aa
Helical: 331-352 aa
Cytoplasmic: 353-771 aa
Helical: 772-732 aa
Extracellular: 733-756 aa
Helical: 757-777 aa
Cytoplasmic: 778-832 aa
Helical: 833-853 aa
Extracellular: 854 aa
Helical: 855-874 aa
Cytoplasmic: 875-934 aa
Helical: 935-957 aa
Extracellular: 958-973 aa
Helical: 974-995 aa
Cytoplasmic: 996-1280 aa
More Infomation

Onuma, S., Manabe, A., Yoshino, Y., Matsunaga, T., Asai, T., & Ikari, A. (2021). Upregulation of Chemoresistance by Mg2+ Deficiency through Elevation of ATP Binding Cassette Subfamily B Member 1 Expression in Human Lung Adenocarcinoma A549 Cells. Cells, 10(5), 1179.

Zhang, L., Graf, I., Kuang, Y., Zheng, X., Haupt, M., Majid, A., ... & Doeppner, T. R. (2021). Neural progenitor cell-derived extracellular vesicles enhance blood-brain barrier integrity by NF-κB (Nuclear Factor-κB)-dependent regulation of ABCB1 (ATP-Binding Cassette Transporter B1) in stroke mice. Arteriosclerosis, thrombosis, and vascular biology, 41(3), 1127-1145.

Zhang, M., Chen, X. Y., Dong, X. D., Wang, J. Q., Feng, W., Teng, Q. X., ... & Chen, Z. S. (2020). NVP-CGM097, an HDM2 inhibitor, antagonizes ATP-binding cassette subfamily B member 1-mediated drug resistance. Frontiers in Oncology, 10, 1219.

Wang, H., Li, J. M., Wei, W., Yang, R., Chen, D., Ma, X. D., ... & Wang, B. L. (2020). Regulation of ATP‐binding cassette subfamily B member 1 by Snail contributes to chemoresistance in colorectal cancer. Cancer science, 111(1), 84-97.

Yuan, J., Xiao, C., Lu, H., Yu, H., Hong, H., Guo, C., & Wu, Z. (2020). Effect of miR-12136 on Drug Sensitivity of Drug-Resistant Cell Line Michigan Cancer Foundation-7/Doxorubicin by Regulating ATP Binding Cassette Subfamily B Member 1. Journal of Biomaterials and Tissue Engineering, 10(10), 1431-1435.

Yang, Y., Ji, N., Cai, C. Y., Wang, J. Q., Lei, Z. N., Teng, Q. X., ... & Chen, Z. S. (2020). Modulating the function of ABCB1: in vitro and in vivo characterization of sitravatinib, a tyrosine kinase inhibitor. Cancer Communications, 40(7), 285-300.

Wang, W., Zhou, Q., & Yuan, C. (2019). Influence of (ATP)-Binding Cassette Transporter Subfamily B Member 1 (ABCB1) Gene Polymorphism on the Efficacy of Remifentanil. Medical science monitor: international medical journal of experimental and clinical research, 25, 5258.

Rastgar‐Moghadam, A., Mehramiz, M., Shahidsales, S., Entezari, M., Hassanian, S. M., Talebian, S., ... & Avan, A. (2019). Association of a genetic variant in ATP‐binding cassette sub‐family B member 1 gene with poor prognosis in patients with squamous cell carcinoma of the esophagus. IUBMB life, 71(9), 1252-1258.

Onodera, M., Endo, K., Kakuta, Y., Kuroha, M., Kimura, T., Hiramoto, K., ... & Shimosegawa, T. (2017). ATP‐binding cassette subfamily B member 1 1236C/T polymorphism significantly affects the therapeutic outcome of tacrolimus in patients with refractory ulcerative colitis. Journal of gastroenterology and hepatology, 32(9), 1562-1569.

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For research use only. Not intended for any clinical use.

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