Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Human AKT Pathway Phosphorylation Antibody Array (Membrane, 18 Targets) (Y0389) (AbAr-0389-YC)


See all AKT Pathway Phosphorylation antibodies
Online Inquiry

Specifications

Target Array
AKT Pathway Phosphorylation Array
Target
Akt (pSer473) (PKB (pSer473)), AMPKa (pThr172), BAD (pSer112), 4E-BP1 (pThr36), ERK1 (pThr202, pTyr204)/ERK2 (pTyr185, pTyr187), GSK3a (pSer21), GSK3b (pSer9), mTOR (pSer2448), p27 (pThr198), P53 (pSer15), P70S6K (pThr421, pSer424), PDK1 (pSer241), PRAS40 (pThr246), PTEN (pSer380), Raf-1 (Ser301), RPS6 (pSer235, pSer236), RSK1 (pSer380), RSK2 (pSer386)
Species Reactivity
Human, Mouse
Sample Type
Cell culture supernatants, Cell lysates, Plasma, Serum and Tissue lysates

Applications

Application
Multiplexed Protein Detection
Assay Type
Semi-Quantitative
Solid Support
Membrane
Detection Assay
Sandwich-based Assay
Protocol Outline
Perform blocking of the array membrane surface.
Incubate with the sample.
Incubate using the detection antibody cocktail.
Proceed with incubation using HRP-anti-rabbit IgG antibodies.
Engage in incubation with detection buffers.
Acquire images with a chemiluminescent imaging system.
Conduct a comprehensive data analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Washing Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at -20°C for 6 months. Once thawed, please keep reagents under suitable conditions respectively for no more than 3 months. Do not use past expiration date.

Background

Introduction
The Akt Pathway, or PI3K-Akt Pathway is a signal transduction pathway that promotes survival and growth in response to extracellular signals. Key proteins involved are PI3K (phosphatidylinositol 3-kinase) and Akt (Protein Kinase B).
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Human AKT Pathway Phosphorylation Antibody Array (Membrane, 18 Targets) (Y0389)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:

Submit A Review

Online Inquiry