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Rabbit Anti-AKT1/AKT2/AKT3 (Phosphorylated T308, T309, T305) Recombinant Antibody (V2-443453) (PTM-CBMAB-0029YC)


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Published Data
Rabbit Anti-AKT1/AKT2/AKT3 (Phosphorylated T308, T309, T305) Recombinant Antibody (244F9) in FC
Intracellular levels of phospho-AKT (Thr308), phospho-ERK1/2 (p44/42 MAPK), phospho-STAT3 (Tyr705), and phospho-STAT5 (Tyr694) in CD34+ HSPCs treated with rhG-CSF or the designs (see the "Methods" section). Geometric mean of the expression intensity of each phospho-protein (GeoMean intensity) is shown on the y-axis. The experiment was performed twice. ...View More
Skokowa, J., Hernandez Alvarez, B., Coles, M., Ritter, M., Nasri, M., Haaf, J., ... & ElGamacy, M. (2022). A topological refactoring design strategy yields highly stable granulopoietic proteins. Nature Communications, 13(1), 2948.
Rabbit Anti-AKT1/AKT2/AKT3 (Phosphorylated T308, T309, T305) Recombinant Antibody (244F9) in IHC
Representative images of nuclear and cytoplasmic protein expression levels in tumor and adjacent normal tissues. The first row shows expression levels in normal tissues. The second, third and fourth row show increasing grades of tumor tissues, including weakly positive (1+), moderately positive (2+) and strongly positive (3+) staining. Magnification, ×40. Scale bar, 60 µm. p, phosphorylated; Ser, serine; Thr, threonine. ...View More
Weng, W. H., Yu, K. J., Li, L. C., Pang, Y. J., Chen, Y. T., Pang, S. T., & Chuang, C. K. (2020). Low PTEN expression and overexpression of phosphorylated Akt Ser473 and Akt Thr308 are associated with poor overall survival in upper tract urothelial carcinoma. Oncology Letters, 20(6), 1-1.
Rabbit Anti-AKT1/AKT2/AKT3 (Phosphorylated T308, T309, T305) Recombinant Antibody (244F9) in IHC
Representative images of PTEN, pAKT and pS6 IHC staining in three patterns of human SACCs. The scale bars represent 100 μm. ...View More
Liu, H., Du, L., Wang, R., Wei, C., Liu, B., Zhu, L., ... & Xiao, J. (2015). High frequency of loss of PTEN expression in human solid salivary adenoid cystic carcinoma and its implication for targeted therapy. Oncotarget, 6(13), 11477.
Rabbit Anti-AKT1/AKT2/AKT3 (Phosphorylated T308, T309, T305) Recombinant Antibody (244F9) in WB
Expression of PI3K/Akt signaling pathway components in 4T1 and 4T1 GPD2 KO cells, as detected by Western blot analysis. ...View More
Oh, S., Jo, S., Bajzikova, M., Kim, H. S., Dao, T. T., Rohlena, J., ... & Park, S. (2023). Non-bioenergetic roles of mitochondrial GPD2 promote tumor progression. Theranostics, 13(2), 438.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
V2-443453
Antibody Isotype
IgG
Application
WB, IP

Basic Information

Immunogen
Synthetic phosphopeptide corresponding to residues around Thr308 of mouse Akt.
Host Species
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 150 mM NaCl, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
AKT1/AKT2/AKT3
Introduction
AKT is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription and cell migration.
Alternative Names
AKT 1; AKT2; AKT3
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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